Genebridege公司的FLPe表達(dá)質(zhì)粒707-710可以促成大腸桿菌中以FLP為媒介的定點重組，在30°C時以低拷貝數(shù)傳播。Flpe基因由λR啟動子驅(qū)動，受控于不耐熱cI857阻遏蛋白。溫度從30°C上升至37°C會引發(fā)FLPe重組酶暫態(tài)表達(dá)。由于pSC101復(fù)制原點的緣故，表達(dá)質(zhì)粒在37°C時不再復(fù)制，因此它們zui終會消失。與野生型FLP蛋白相比，F(xiàn)LPe擁有更好的耐熱性，在37 – 40°C時顯示出更強的重組酶活性

產(chǎn)品信息如下：

All Red/ET Recombination Kits come with tailored control experiments and harbor the same Red/ET proficiency plasmid.

 

For all types of BAC modifications.
ID: K001	690.00 €*	Details	Manual	Order

 

For advanced BAC modifications using a counter-selection cassette, also for bacterial chromosomes.
ID: K002	795.00 €*	Details	Manual	Order

 

For subcloning of DNA fragments from BACs and cosmids.
ID: K003	620.00 €*	Details	Manual	Order

 

For fast and simple integration of FRT-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）.
ID: K004	690.00 €*	Details	Manual	Order

 

For fast and simple integration of loxP-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）.
ID: K005	690.00 €*	Details	Manual	Order

 

For fast knockouts or modifications of genes on the E.coli chromosome.
ID: K006	620.00 €*	Details	Manual	Order

Red/ET Recombination Strains

 

Direct cloning-proficient E. coli strain for linear plus linear homologous recombination
ID: K008	590.00 €*	Details	Manual	Order

 

Recombineering-proficient E. coli strain for linear plus circular homologous recombination
ID: K009	590.00 €*	Details	Manual	Order

Selection Cassettes

Also available are accessory selection cassettes designed for use in Eukaryotes or Prokaryotes, some flanked by loxP- or FRT-sites. These sites will allow the subsequent removal of the selection marker by a Cre- or FLP-recombinase step if required. These cassettes may be very helpful for generating targeting constructs in mammals, or for introducing sequences without leaving a selection marker behind.

 

Pro- and Eukaryotic Neomycin Selection Cassette
ID: A001	290.00 €*	Details	Manual	Order

 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites
ID: A002	290.00 €*	Details	Manual	Order

 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by loxP-sites
ID: A003	290.00 €*	Details	Manual	Order

 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional downstream loxP site
ID: A004	290.00 €*	Details	Manual	Order

 

Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional upstream loxP site
ID: A005	290.00 €*	Details	Manual	Order

 

Prokaryotic Chloramphenicol Selection Cassette flanked by FRT-sites
ID: A006	290.00 €*	Details	Manual	Order

 

Prokaryotic Chloramphenicol Selection Cassette flanked by loxP-sites
ID: A007	290.00 €*	Details	Manual	Order

 

Prokaryotic Ampicillin Selection Cassette flanked by FRT-sites
ID: A008	290.00 €*	Details	Manual	Order

 

Prokaryotic Ampicillin Selection Cassette flanked by loxP-sites
ID: A009	290.00 €*	Details	Manual	Order

 

FRT flanked, Pro- and Eukaryotic Hygromycin Selection Cassette
ID: A010	290.00 €*	Details	Manual	Order

 

loxP flanked, Pro- and Eukaryotic Hygromycin Selection Cassette
ID: A011	290.00 €*	Details	Manual	Order

 

Codon improved Cre （iCre） with attached FRT flanked, Pro- and Eukaryotic Neomycin Selection Cassette
ID: A012	325.00 €*	Details	Manual	Order

 

Expression Plasmids

The following recombinase expression plasmids are very useful to excise FRT- or loxP-flanked DNA stretches （such as selection markers） during a modification task. The plasmids are easily transformed into cells resulting in an activation of the recombinases in vivo.

 

 

Flp Expression Plasmid with tetracycline resistance marker for use in E.coli only
ID: A104	290.00 €*	Details	Manual	Order

 

 

Flp Expression Plasmid with chloramphenicol resistance marker for use in E.coli only
ID: A105	290.00 €*	Details	Manual	Order

 

 

Flp Expression Plasmid with ampicillin resistance marker for use in E.coli only
ID: A106	275.00 €*	Details	Manual	Order

 

 

Flp Expression Plasmid with kanamycin resistance marker for use in E.coli only
ID: A107	275.00 €*	Details	Manual	Order

 

 

Cre Expression Plasmid with chloramphenicol resistance marker for use in E.coli only
ID: A112	290.00 €*	Details	Manual	Order

 

 

Cre Expression Plasmid with tetracycline resistance marker for use in E.coli only
ID: A113	290.00 €*	Details	Manual	Order

 

 

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Academia）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement.
ID: A201	375.00 €*	Details	Manual	Order

 

 

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Industry）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement.
ID: A202	3250.00 €*	Details	Manual	Order