世界*實驗材料供應(yīng)商 Sanquin 正式授權(quán)上海起發(fā)為其中國代理， Sanquin 在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Sanquin 就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Sanquin是通過荷蘭血庫和荷蘭紅十字會輸血服務(wù)中心實驗室之間的合并，成立于1998年（CLB）。公司成立基金會負責的血液供應(yīng)，以非營利為基礎(chǔ)提出輸血等，符合zui高質(zhì)量要求，安全性和效率。Sanquin為科學(xué)研究和提供教育，教學(xué)，培訓(xùn)。Sanquin血液供應(yīng)的基礎(chǔ)保證安全有效的在荷蘭的血液供應(yīng)。Sanquin還開發(fā)并生產(chǎn)醫(yī)藥產(chǎn)品，進行高質(zhì)量的科學(xué)研究，開發(fā)和執(zhí)行多種診斷服務(wù)。

Sanquin human IgG subclass Enzym Linked Immuno Sorbent Assay (ELISA)

Sanquin’s enzyme-linked immunosorbent assay is a sandwich type immunoassay. The kit contains microwell strips coated with highly avid monoclonal antibodies.

Principle

Test samples, calibrator and control sera are incubated in the wells. The IgG subclass to be determined binds to the solid phase and non-bound IgG is removed by washing.

Next peroxidase-conjugated anti-human IgG antiserum is added and non-bound conjugate is removed by washing. After incubation with substrate solution (ABTS) and H₂O₂ the reaction is stopped with an acid buffer.

The green coloured reaction product is measured by absorption and the concentration of the IgG subclass is calculated relatively to the values of a reference curve.

The IgG subclass concentrations in the calibrators were determined using a calibrator derived from the WHO 67/97 reference preparation.

Content of the ELISA kit

The kit contains 48 tests, including calibrators and controls, for each of the four subclasses:
• 2 precoated microtiter plates
• HRP conjugated a-IgG antibodies
• standard and control sera
• wash, dilution, substrate and stop buffers
• ABTS and hydrogen peroxidase substrate

Product number of the kit is M1551.
510(k) number: 943098