世界*實驗材料供應商 ReliaTech GmbH正式授權上海起發(fā)為其中國代理， ReliaTech GmbH在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務,上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務，簽約 ReliaTech GmbH就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務,您的滿意將是我們zui大的收獲

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ReliaTech GmbH （Receptor Ligand Technologies GmbH的簡稱）是后基因組德國生物技術公司成立于1999。除了研究公司的產(chǎn)品開發(fā)，生產(chǎn)和銷售高質量的血管生成和淋巴管的研究研究試劑。
作為一個有自己內部的試劑生產(chǎn)reliatech GmbH公司還提供為客戶特定的試劑生產(chǎn)解決方案。我們在蛋白質純化方面的深刻經(jīng)驗和日常實驗室業(yè)務的實踐經(jīng)驗也滲透到我們的基于客戶的項目中，使我們成為需要單個生產(chǎn)解決方案的項目的合格合作伙伴。

Bovine ECGF (Crude Extract, cell culture grade) + Heparin

Size:   6 mg

Formulation:  Crude extract ; lyophilized (freeze-dried)

Catalog Number:  300-090H

Synonyms:  Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS)

Application:  cell culture

Biological activity:  Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 µg/ml, optimal concentration with heparin (50 µg/ml) is about 12 µg/ml.

Reactivity:  human cells, mouse cells, cattle cells, pig cells

Stability:  Also stable at 4 °C for several weeks it is recommended to store the product below 0 °C. After reconstitution the product shout be stored in aliquots at -20 °C to -70 °C.

Reconstitution:  Reconstitute the contents of the vial in 2 ml of prewarmed (37 °C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.

Remarks:   BSE-free tested!

Description:  Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to c*te endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

相關產(chǎn)品如下：

我們公司zui大優(yōu)勢是強大的采購，

1：基本什么都能進口，血清，抗體，耗材，還有部分限制進口的，

2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，

3：提供加急服務，一般1-2周到貨，超過時限加急費全免

4：價格公道，絕大部分價格有優(yōu)勢，當然不能保證100%產(chǎn)品都是,因為意味著沒有服務.

5：良好的信譽，大部分客戶我們提供貨到付款服務，客戶包括清華，北大 交大 復旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc.  Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。