RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter （P_U6; RNA Pol III-dependent）. RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.